The Ukrainian Biochemical Journal
ISSN 2409-4943

Journal archive > 2015 > N 1 January-February

ROLE OF Ca IONS IN THE INDUCTION OF HEAT-RESISTANCE
OF WHEAT COLEOPTILES BY BRASSINOSTEROIDS 

 Yu. E. Kolupaev1, A. A. Vayner1, T. O. Yastreb1, A. I. Oboznyi1, V. A. Khripach2

1V. V. Dokuchaev Kharkiv National Agrarian University, Ukraine;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.;
2Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus;
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The involvement of Ca2+ into the signal transduction of exogenous brassinosteroids (BS) (24-epibrassinolide – 24-EBL and 24-epicastasterone – 24 ECS) causing the increase of heat resistance of the cells of wheat (Triticum aestivum L.) coleoptiles was investigated using calcium chelator EGTA and inhibitor of phosphatidylinositol-specific phospholipase C – neomycin. Twenty-four-hour treatment of coleoptile segments with 10 nM solutions of 24-EBL and 24-ECS led to a transient increase in the generation of superoxide anion radical by cell surface and the subsequent activation of superoxide dismutase and catalase. Pretreatment of coleoptiles with EGTA and neomycin depressed to a considerable extent these effects and leveled the increase in heat resistance of wheat coleoptiles that were caused by BS. Possible mechanisms of involvement of calcium signaling into the formation of reactive oxygen species in plant cells and induction of heat resistance of plant cells by the action of exogenous BS have been discussed.

Key words: brassinosteroids, phospholipase C, reactive oxygen species, calcium, antioxidant enzymes, Triticum aestivum L.

The original article in Russian is available for download in PDF format.

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