ISSN 2409-4943
Ukr. Biochem. J.

Journal archive > 2014 > N 6 November-December

KINETIC PROPERTIES OF ADENOSINE TRIPHOSPHATE SULFURYLASE
OF INTESTINAL SULFATE-REDUCING BACTERIA

I. V. Kushkevych1,2, H. L. Antonyak3, M. Bartoš2

1Institute of Animal Biology, NAAS of Ukraine, Lviv;
2University of Veterinary and Pharmaceutical Sciences of Brno, Czech Republic;
3Ivan Franko National University of Lviv, Ukraine;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The investigation of specific activity of ATP sulfurylase and kinetic properties of the enzyme in cell-free extracts of intestinal bacterial strains Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 is presented. The microbiological, biochemical, biophysical and statistical methods were used in the work. The optimal temperature (35 ºC) and pH 8.0-8.5 for enzyme reaction were determined. An analysis of kinetic pro­perties of ATP sulfurylase has been carried out. Initial (instantaneous) reaction velocity (V0), maximum amount of the product of reaction (Pmax), the reaction time (half saturation period, τ) and maximum veloci­ty of the ATP sulfurylase reaction (Vmax) have been defined. Michaelis constants (KmSulfate, KmATP, KmAPS, and KmPyrophosphate­) of the enzyme reaction were demonstrated for both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains.

Key words: kinetic analysis, sulfate-reducing bacteria, Desulfovibrio piger, Desulfomicrobium sp., ATP sulfurylase, intestinal microbiocenosis, inflammatory bowel diseases, ulcerative colitis.

The original article in English is available for download in PDF format.

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