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Journal archive > 2014 > N 3 May-June

BIOPHYSICAL PARAMETERS OF ERYTHROCYTE MEMBRANES AND MECHANISMS
OF INTERACTION WITH NON-OPIOID ANALGESICS UNDER ACUTE PAIN SYNDROME
 
Yu. I. Gubskyi1, T. A. Buhtiarova1, H. G. Goriushko1, N. V. Litvinova1, G. I. Paramonova2,
T. N. Kurapova1, O. N. Velychko1, L. P. Babenko1
 
1SI Institute of Pharmacology and Toxicology, National Academy of Medical Sciences of Ukraine, Kyiv;
2SI D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine, Kyiv;

Methods of fluorescent probing, spectrophotometry and microcalorimetry were applied to investigate the alterations in biophysical parameters of erythrocytes membranes, and specifically microviscosity, surface charge, molecular organization of lipid bilayer and lipid-protein interactions under conditions of acute pain syndrome produced by experimental chemical lesion. The distinctive features of non-opiod analgesics interactions and binding to the erythrocytes membranes of rats subjected to acute nociceptive pain accompanied with oxidative stress development were investigated. The abilities of analgesics under research, and namely paracetamol, aspirin, phenazone, ketorolac, pyrodazole, ketoprofenum, natrium mefenaminate, indometacin, nimesulide to make up physico-chemical complexes with lipoperoxidation modified erythrocytes surface and protein-lipid bilayer showed marked changes. The significance of oxidative damage of biophase under conditions of acute pain syndrome for analgesics effective pharmacodynamics and pharmacokinetics realization is under consideration.

Key words: acute nociceptive pain,oxidative stress, non-opioid analgesics, erythrocytes membranes, fluorescent probing, microcalorimetry.

The original article in Ukrainian is available for download in PDF format.

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