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Journal archive > 2014 > N 1 January-February

ESTIMATION OF ATP-DEPENDENT K+-CHANNEL CONTRIBUTION TO POTENTIAL-DEPENDENT
POTASSIUM UPTAKE IN THE RAT BRAIN MITOCHONDRIA

O. V. Akopova, V. I. Nosar, L. I. Kolchinskaya, I. N. Mankovska, V. F. Sagach

Bogomoletz Institute of  Physiology, National Academy of Sciences of Ukraine, Kyiv;

The effect of potassium on state 4 respiration (substrate oxidation in the absence of ADP) was investigated. It was shown that potential-dependent potassium uptake in the brain mitochondria results in mitochondrial depolarization. Taking into account depolarization effect of potassium, the contribution of the endo­genous proton leak as well as K+-uptake to the re­spiration rate was calculated. It was shown that such estimation allows the share of ATP-dependent potassium channel contribution to potential-dependent potassium uptake to be determined by polarographic method.

Key words: K+-uptake, respiration, H+-leak, membrane potential, K+ATP-channel, brain mitochondria.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal