Journal archive > 2014 > N 1 January-February
O. V. Akopova, V. I. Nosar, L. I. Kolchinskaya, I. N. Mankovska, V. F. Sagach
The effect of potassium on state 4 respiration (substrate oxidation in the absence of ADP) was investigated. It was shown that potential-dependent potassium uptake in the brain mitochondria results in mitochondrial depolarization. Taking into account depolarization effect of potassium, the contribution of the endogenous proton leak as well as K+-uptake to the respiration rate was calculated. It was shown that such estimation allows the share of ATP-dependent potassium channel contribution to potential-dependent potassium uptake to be determined by polarographic method.
Key words: K+-uptake, respiration, H+-leak, membrane potential, K+ATP-channel, brain mitochondria.
The original article in Russian is available for download in PDF format.
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