ESTIMATION OF ATP-DEPENDENT K+-CHANNEL CONTRIBUTION TO POTENTIAL-DEPENDENT POTASSIUM UPTAKE IN THE RAT BRAIN MITOCHONDRIA (O.V. Akopova, V.I. Nosar, L.I. Kolchinskaya, I.N. Mankovska, V.F. Sagach)

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Journal archive > 2014 > N 1 January-February

ESTIMATION OF ATP-DEPENDENT K⁺-CHANNEL CONTRIBUTION TO POTENTIAL-DEPENDENT

POTASSIUM UPTAKE IN THE RAT BRAIN MITOCHONDRIA

O. V. Akopova, V. I. Nosar, L. I. Kolchinskaya, I. N. Mankovska, V. F. Sagach

Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;

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The effect of potassium on state 4 respiration (substrate oxidation in the absence of ADP) was investigated. It was shown that potential-dependent potassium uptake in the brain mitochondria results in mitochondrial depolarization. Taking into account depolarization effect of potassium, the contribution of the endogenous proton leak as well as K⁺-uptake to the respiration rate was calculated. It was shown that such estimation allows the share of ATP-dependent potassium channel contribution to potential-dependent potassium uptake to be determined by polarographic method.

Key words: K⁺-uptake, respiration, H⁺-leak, membrane potential, K⁺_ATP-channel, brain mitochondria.

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