Journal archive > 2014 > N 1 January-February
S. G. Shlykov, L. G. Babich, M. E. Yevtushenko, S. O. Karakhim, S. O. Kosterin
Influence of calmodulin antagonists on mitochondrial membrane potential was investigated using a flow cytometry method, confocal microscopy and fluorescent potential-sensitive probes TMRM and MTG. Influence of different concentrations of calmodulin antagonists on mitochondrial membrane potential was studied using flow cytometry method and a fraction of myometrium mitochondria of unpregnant rats. It was shown that 1-10 µМ calmidazolium gradually reduced mitochondria membrane potential. At the same time 10-100 µМ trifluoperazine influenced as follows: 10 µМ – increased polarization, while 100 µМ – caused almost complete depolarization of mitochondrial membranes. In experiments which were conducted with the use of confocal microscopy method and myometrium cells it was shown, that MTG addition to the incubation medium led to the appearance of fluorescence signal in a green range. Addition of the second probe (ТМRM) resulted in the appearance of fluorescent signal in a red range. Mitochondrial membrane depolarization by 1µМ СССР or 10 mМ NaN3 was accompanied by the decline of “red” fluorescence intensity, “green” fluorescence was kept. The 10-15 minute incubation of myometrium cells in the presence 10 µМ calmidazolium or 100 µМ trifluoperazine was accompanied by almost complete decrease of the TMRM fluorescent signal. Thus, with the use of potential-sensitive fluorescent probes TMRM and MTG it was shown, that calmodulin antagonists modulate mitochondrial membrane potential of myometrium cells.
Key words: flow cytometry, confocal fluorescence microscopy, isolated mitochondria, smooth muscles cells, mitochondrial membrane potential, calmidazolium, trifluoperazine.
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