INVESTIGATION OF NITROSACTIVE COMPOUNDS INFLUENCE ON POLARIZATION OF THE MITOCHONDRIAL INNER MEMBRANE IN THE RAT UTERUS MYOCYTES USING POTENTIAL SENSITIVE FLUORESCENT PROBE DіOC6(3) (Yu. V. Danylovych, H. V. Danylovych, O. V. Kolomiets, S. O. Kosterin...)

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Journal archive > 2014 > N 1 January-February

INVESTIGATION OF NITROSACTIVE COMPOUNDS INFLUENCE ON POLARIZATION

OF THE MITOCHONDRIAL INNER MEMBRANE IN THE RAT UTERUS MYOCYTES USING POTENTIAL

SENSITIVE FLUORESCENT PROBE DіOC₆(3)

Yu. V. Danylovych, H. V. Danylovych, O. V. Kolomiets, S. O. Kosterin, S. A. Karakhim, A. Yu. Chunikhin

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;

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The effect of nitrosactive compounds (sodium nitroprusside and sodium nitrite) on the polarization level of the uterus myocytes inner mitochondrial membrane using the confocal laser microscopy and fluorescent probe potentialsensitive DiOC₆(3) (3,3?-dihexyloxacarbocyanine) was ivestigated. Colocalisation of mitochondrial membranes specific fluorescent probes (MitoTracker Orange CM H₂TMRos, 10 – nonyl acridine orange and DiOC₆(3)) was demonstrated. It was shown that sodium nitroprusside at 0.1 mM concentration caused a moderate decrease in mitochondrial transmembrane potential. That observation was confirmed by flow cytometry. Action efficiency of sodium nitrite in a similar concentration was significantly lower than that of sodium nitroprusside. It is shown that it was sodium nitroprusside which caused a slight swelling of the mitochondria. A possible protecting role of nitric oxide as to mitochondria was discussed.

Key words: mitochondria, nitric oxide, transmembrane potential, myometrium, laser confocal microscopy.

The original article in Ukrainian is available for download in PDF format.