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Journal archive > 2014 > N 1 January-February

ТHE MASS-SPECTROMETRY STUDIES OF THE INTERACTION
F POLYHEXAMETHYLENEGUANIDINE WITH LIPIDS

A. V. Lysytsya1, A. V. Rebriev2

1Agriculture Institute of the West Polissya, National Academy of Agrarian Sciences of Ukraine, Rivne;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;

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In this work the integral components of the cytoplasmic membrane, lecithin and cholesterol were used for mass spectrometry analysis carried out on polyhexamethyleneguanidine (PHMG) mixtures with lipids. The study was performed by mass-spectrometry methods of the MALDI-TOF MS. Our results showed that despite the common use of PHGM polymer derivatives as disinfectants the persistent intermolecular complexes of PHMG oligomers with lipids were not formed. The binding of polycation PHMG with the membrane has been explained by the model proposed. According to this model PHGM can adhere to negatively charged plasma membrane of bacterial cell due to electrostatic interaction and the formation of loop-like structures. Similar stereochemistry mechanism makes the adsorption of the investigated polycation to membrane robust. The mechanism described together with additional destructive factors provides a reasonable explanation for the PHMG induced damage of bacterial cell plasma membrane and the biocide action of disinfectants prepared on the basis of the PНMG salts.

Key words: polyhexamethyleneguanidine, disinfectant, membrane destruction, lipids, mass-spectrometry.

The original article in Ukrainian is available for download in PDF format.

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