title en

Journal archive > 2014 > N 1 January-February

INTERACTION PECULIARITIES OF POLYREACTIVE IMMUNOGLOBULINS AND VARIOUS ANTIGENS

S. A. Bobrovnik, M. O. Demchenko, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukarine, Kyiv;

The influence of twin 20, lysozyme and protamine on the capability of polyreactive immunoglobu­lins (PRIG) to attach to various antigens was investigated. Twin 20 can inhibit the binding of PRIG to antigens on immunological plates but lysozyme and protamine can enhance it. As far as the mixture of the optimal concentrations of lysozyme and protamine cannot increase PRIG-antigen interaction in comparison to the optimal dose of protamine, we have concluded that the mechanism of their effect on PRIG binding is similar. Of special interest­ is the fact that twin 20 at optimal concentration of lysozyme or protamine does not decrease PRIG binding to various antigens but, on the contrary, increases PRIG-antigen interaction.

Key words: polyreactive immunoglobulins, antigen-antibody interaction, lysozyme, protamine, twin 20, ELISA.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal