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Journal archive > 2014 > N 5 September-October  

MULTIPLE MOLECULAR FORMS OF ADAPTOR PROTEIN RUK/CIN85 SPECIFICALLY
ASSOCIATE WITH DIFFERENT SUBCELLULAR COMPARTMENTS IN HUMAN
BREAST ADENOCARCINOMA MCF-7 CELLS
B. O. Vynnytska-Myronovska1, Ya. P. Bobak1, G. V. Pasichnyk2, N. I. Igumentseva1,
A. A. Samoylenko2, L. B. Drobot2

 

1Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Ruk/CIN85 is a receptor-proximal ‘signalling’ adaptor that possesses three SH3 domains, Pro- and Ser-rich regions and C-terminal coiled-coil domain. It employs distinct domains and motifs to act as a transducer platform in intracellular signalling. Based on cDNA analysis, various isoforms of Ruk/CIN85 with different combination of protein-protein interaction domains as well as additional Ruk/CIN85 forms that are the products of post-translational modifications have been demonstrated. Nevertheless, there is no precise information regarding both the subcellular distribution and the role of Ruk/CIN85 multiple molecular forms in cellular responses. Using MCF-7 human breast adenocarcinoma cells and cell fractionation technique, specific association of Ruk/CIN85 molecular forms with different subcellular compartments was demonstrated. Induction of apoptosis of MCF-7 cells by doxorubicin treatment or by serum deprivation resulted in the system changes of Ruk/CIN85 molecular forms intracellular localization as well as their ratio. The data obtained provide a new insight into potential physiological significance of Ruk/CIN85 molecular forms in the regulation of various cellular functions.

Key words: adaptor proteins, Ruk/CIN85, multiple molecular forms, MCF-7 cells, subcellular distribution, apoptosis.

The original article in English is available for download in PDF format.

© The Ukrainian Biochemical Journal