# 5 September-October (2014)-en

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Journal archive > 2014

Vol. 86, N 5 September-October, 2014

Review

Zaichko N. V., Melnik A. V., Yoltukhivskyy M. M., Olhovskiy A. S., Palamarchuk I. V.

HYDROGEN SULFIDE: METABOLISM, BIOLOGICAL AND MEDICAL ROLE

Mokrosnop V. M.

FUNCTIONS OF TOCOPHEROLS IN THE CELLS OF PLANTS AND OTHER PHOTOSYNTHETIC ORGANISMS

Experimental Works

Veklich T. O., Shkrabak A. A., Mazur Yu. Yu., Rodik R. V., Kalchenko V. I., Kosterin S. O.

KINETICS OF INHIBITORY EFFECT OF CALIX[4]ARENE С-90 ON ACTIVITY OF TRANSPORTING PLASMA MEMBRANE Cа²⁺, Mg²⁺-ATPase OF SMOOTH MUSCLE CELLS

Basova N. E., Kormilitsyn B. N., Perchenok A. Yu., Rozengart E. V., Saakovv. S., Suvorov A. A.

INHIBITORY EFFECT OF BENZIMIDAZOLE DERIVATIVES ON CHOLINESTERASES OF ANIMALS IN THE PRESENCE OF DIFFERENT SUBSTRATES

Tseyslyer Yu. V., Podpalova О. M., Nurishchenko N. Е., Маrtyniuk V. S.

ACTOMYOSIN ATPase ACTIVITY OF SKELETAL MUSCLES AND THE MARKERS OF TISSUE DAMAGE IN THE BLOOD OF RATS UNDER PROLONGED CHRONIC ALCOHOLIZATION

Bychkova S. V., Chorna T. I.

NAADP-SENSITIVE Сa²⁺ STORES IN PERMEABILIZED RAT HEPATOCYTES

Tykhomyrov A. A.

DYNAMICS OF THROMBIN-INDUCED EXPOSITION OF ACTIN ON THE PLATELET SURFACE

Roka-Moya Y. M., Zhernossekov D. D., Yusova E. I., Kapustianenko L. G., Grinenko T. V.

STUDY OF THE SITES OF PLASMINOGEN MOLECULE WHICH ARE RESPONSIBLE FOR INHIBITORY EFFECT OF Lys-PLASMINOGEN ON PLATELET AGGREGATION

Martynenko O. I., Kyrylenko T. K., Zaimenko N. V., Antonyuk M. M., Stepanyugin A. V., Plodnik D. P., Hovorun D. M.

RELATIONSHIP BETWEEN RNA/DNA RATIO, GROWTH RATE AND ACCUMULATION OF SELENIUM IN THE CELLS OF WHEAT LEAVES UNDER THE INFLUENCE OF MINERALS ANALCIME AND TREPEL

Kit Yu. Ya., Myronovsky S. L., Kril’ I. I., Havrylyuk A. M., Chop’yak V. V., Stoika R. S.

PROTEOLYTIC ACTIVITY OF IgGs FROM BLOOD SERUM OF WISTAR RATS AT EXPERIMENTAL RHEUMATOID ARTHRITIS

Vynnytska-Myronovska B. O., Bobak Ya. P., Pasichnyk G. V., Igumentseva N. I., Samoylenko A. A., Drobot L. B.

MULTIPLE MOLECULAR FORMS OF ADAPTOR PROTEIN Ruk/CIN85 SPECIFICALLY ASSOCIATE WITH DIFFERENT SUBCELLULAR COMPARTMENTS IN HUMAN BREAST ADENOCARCINOMA MCF-7 CELLS

Shymanskyy I. O., Khomenko A. V., Lisakovska O. O., Labudzynskyi D. O., Apukhovska L. I., Veliky M. M.

THE ROS-GENERATING AND ANTIOXIDANT SYSTEMS IN THE LIVER OF RATS TREATED WITH PREDNISOLONE AND VITAMIN D₃

Ryzhko I. L., Motruk N. V., Petrov S. A.

INFLUENCE OF HEAVY METAL SALTS ON THE ACTIVITY OF TRYPSIN-LIKE HYDROLASES FROM Drosophila melanogaster

Kovalenko N. O., Bilyk Zh. I., Palladina T. A.

EFFECT OF ADAPTOGENIC PREPARATIONS ON Na⁺/H⁺-ANTIPORTER FUNCTION IN PLASMA MEMBRANE OF CORN ROOT CELLS UNDER SALINITY CONDITIONS

Yaremchuk M. M., Dyka M. V., Sanagursky D. I.

THE ACTIVITY OF PROOXIDANT-ANTIOXIDANT SYSTEM IN LOACH EMBRYOS UNDER THE ACTION OF MICROWAVE RADIATION

Brief Notes

Bobrovnik S. A., Demchenko M. A., Komisarenko S. V.

AGE CHANGES OF HUMAN SERUM POLYREACTIVE IMMUNOGLOBULINS (PRIG) ACTIVITY

The History of Biochemistry

Vynogradova R. P., Danilova V. M.

LAUREATES OF THE PALLADIN PRIZE OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE (2005, 2007 YEARS)

News Items

Danilova V. M., Vynogradova R. P., Komisarenko S. V.

IVAN YAKOVYCH GORBACHEVSKY – SCIENTIST, PATRIOT, CITIZEN

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Journal archive > 2014 > N 5 September-October

AGE CHANGES OF HUMAN SERUM POLYREACTIVE IMMUNOGLOBULINS (PRIG) ACTIVITY

S. A. Bobrovnik, M. A. Demchenko, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;

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It has been determined that activity of serum polyreactive immunoglobulins (PRIG) changes with age in practically healthy people at the age from twenty five to seventy. Therewith, the activity of serum IgG PRIG increases most of all (about 3-4 times), while IgM PRIG activity, on the contrary, does not increase, but sometimes even decreases with age. IgA PRIG activity varies significantly more than IgG PRIG activity and, besides, IgА PRIG significantly less depends on age than IgG PRIG. The age changes in the activity of human serum PRIG, belonging to different types of immunoglobulins, may evidence for the important functional role of these immunoglobulins that has to be clarified.

Key words: activity of polyreactive IgG, age changes, ELISA.

The original article in English is available for download in PDF format.

title en

Journal archive > 2014 > N 5 September-October

EFFECT OF ADAPTOGENIC PREPARATIONS ON Na⁺/H⁺-ANTIPORTER FUNCTION

IN PLASMA MEMBRANE OF CORN ROOT CELLS UNDER SALINITY CONDITIONS

N. O. Kovalenko, Zh. I. Bilyk, T. A. Palladina

Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv;

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Salinity is a hard stress factor for plant organisms which negative effect is caused chiefly by sodium toxic for plants. Plant cells try to remove Na⁺ from their cytoplasm outside and to vacuolar space by secondary active Na⁺/H⁺-antiporters. Their functions can be intensified by gene engineering methods however we try do it with the help of non-toxic bioactive preparations. A comparison of their effect on the plasma membrane of Na⁺/H⁺-antiporters was carried out on corn seedling roots of Zea mays L. exposed at 0.1 M NaCl. Before we have established that Methyure used by seed pretreating possesses a high salt protective ability as against Ivine. It was found that without NaCl exposition Na⁺/H⁺-antiporter activity in root plasma membrane was nearly unnoticeable but increased slightly with seedling age. Methyure and Ivine did not influence its activity in control root seedling. One day 0.1 M NaCl exposition evoked a considerable increasing of Na⁺/H⁺-antiporter activity and its gene expression but these effects disappeared at 10 day NaCl exposition. Methyure use reinforced Na⁺/H⁺-antiporter activity and prolonged it at NaCl exposition without effect on its gene expression whereas Ivine effects on these indexes were insignificant. Obtained results showed that the salt protective capability of Methyure is connected with plasma membrane Na⁺/H⁺-antiporter activation which is realized on molecular level.

Key words: Zea mays L., plasma membrane, salt stress, Na⁺/H⁺-antiporter, Methyure, Ivine.

The original article in Ukrainian is available for download in PDF format.

Journal archive > 2014 > N 5 September-October

THE ACTIVITY OF PROOXIDANT-ANTIOXIDANT SYSTEM IN LOACH EMBRYOS

UNDER THE ACTION OF MICROWAVE RADIATION

M. M. Yaremchuk, M. V. Dyka, D. I. Sanagursky

Ivan Franko National University of Lviv,Ukraine;

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Electromagnetic radiation (EMR) affects biological organisms, primarily on the cellular level. However, the effects of EMR at low-intensity exposure on animals and state of metabolic systems are not fully defined yet. Thus, research of microwave radiation influence on the processes of lipid peroxidation and antioxidant protection system is important for understanding the mechanisms of EMR action on the cell, in particular, and organism development on the whole. The content of lipid peroxidation products – lipid hydroperoxides, thiobarbituric acid reactive substances and the activity of antioxidant enzymes – superoxide dismutase, glutathione peroxidase and catalase in loach embryos under the action of microwave radiation (GSM-900 MHz, SAR = 1.1 Vt/kg) lasting 1; 5; 10 and 20 min during early embryogenesis were studied. It has been found that content of lipid peroxidation products in germ cells undergoes significant changes under the action of low-intensity EMR. The effect of microwave radiation (1, 5, 10 min) leads to the increase of superoxide dismutase activity, nevertheless, 20 min exposure decreased this index to the level of control values as it is shown. It has been established that EMR at frequencies used for mobile communications reduce the activity of antioxidant protection system components, especially catalase and glutathione peroxidase. The growth of catalase activity at the 10-cell stage of blastomere division (P < 0.05) is an exception. The results of two-way analysis of variance attest that microwave radiation factor causes the large part of all observable modifications.

Key words: microwave radiation, loach embryos, lipid peroxidation, superoxide dismutase, catalase, glutathione peroxidase.

The original article in Ukrainian is available for download in PDF format.

title en

Journal archive > 2014 > N 5 September-October

INFLUENCE OF HEAVY METAL SALTS ON THE ACTIVITY OF TRYPSIN-LIKE

HYDROLASES FROM Drosophila melanogaster

I. L. Ryzhko, N. V. Motruk, S. A. Petrov

Odessa I. I. Mechnikov National University, Ukraine;

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The influence of salts of heavy metals on trypsin-like peptide hydrolase of drosophila larvae partly refined by methods of salting-out, gel chromatography and electrophoresis has been researched. It is established that cadmium chloride is characterized by the greatest inhibitory effect, while zinc chloride by the lowest one. Since metal chlorides were used in all cases, it is the differentiated effect of metal ions on manifestations of amidase activity of trypsin-like peptide hydrolase of drosophila larvae, which rather may be considered as proved than the effect of chlorine ions. This, as a whole, agrees with the effect of these ions on proteolytic digestion system at the level of live organisms.

Key words: heavy metals, peptide hydrolyse, drosophila.

The original article in Ukrainian is available for download in PDF format.

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