Journal archive > 2014
Vol. 86, N 5 September-October, 2014
Review
Experimental Works
Brief Notes
The History of Biochemistry
News Items
© The Ukrainian Biochemical Journal
Journal archive > 2014
Vol. 86, N 5 September-October, 2014
Review
Experimental Works
Brief Notes
The History of Biochemistry
News Items
© The Ukrainian Biochemical Journal
Journal archive > 2014 > N 5 September-October
AGE CHANGES OF HUMAN SERUM POLYREACTIVE IMMUNOGLOBULINS (PRIG) ACTIVITY
S. A. Bobrovnik, M. A. Demchenko, S. V. Komisarenko
It has been determined that activity of serum polyreactive immunoglobulins (PRIG) changes with age in practically healthy people at the age from twenty five to seventy. Therewith, the activity of serum IgG PRIG increases most of all (about 3-4 times), while IgM PRIG activity, on the contrary, does not increase, but sometimes even decreases with age. IgA PRIG activity varies significantly more than IgG PRIG activity and, besides, IgА PRIG significantly less depends on age than IgG PRIG. The age changes in the activity of human serum PRIG, belonging to different types of immunoglobulins, may evidence for the important functional role of these immunoglobulins that has to be clarified.
Key words: activity of polyreactive IgG, age changes, ELISA.
The original article in English is available for download in PDF format.
© The Ukrainian Biochemical Journal
Journal archive > 2014 > N 5 September-October
N. O. Kovalenko, Zh. I. Bilyk, T. A. Palladina
Salinity is a hard stress factor for plant organisms which negative effect is caused chiefly by sodium toxic for plants. Plant cells try to remove Na+ from their cytoplasm outside and to vacuolar space by secondary active Na+/H+-antiporters. Their functions can be intensified by gene engineering methods however we try do it with the help of non-toxic bioactive preparations. A comparison of their effect on the plasma membrane of Na+/H+-antiporters was carried out on corn seedling roots of Zea mays L. exposed at 0.1 M NaCl. Before we have established that Methyure used by seed pretreating possesses a high salt protective ability as against Ivine. It was found that without NaCl exposition Na+/H+-antiporter activity in root plasma membrane was nearly unnoticeable but increased slightly with seedling age. Methyure and Ivine did not influence its activity in control root seedling. One day 0.1 M NaCl exposition evoked a considerable increasing of Na+/H+-antiporter activity and its gene expression but these effects disappeared at 10 day NaCl exposition. Methyure use reinforced Na+/H+-antiporter activity and prolonged it at NaCl exposition without effect on its gene expression whereas Ivine effects on these indexes were insignificant. Obtained results showed that the salt protective capability of Methyure is connected with plasma membrane Na+/H+-antiporter activation which is realized on molecular level.
Key words: Zea mays L., plasma membrane, salt stress, Na+/H+-antiporter, Methyure, Ivine.
The original article in Ukrainian is available for download in PDF format.
© The Ukrainian Biochemical Journal
M. M. Yaremchuk, M. V. Dyka, D. I. Sanagursky
Electromagnetic radiation (EMR) affects biological organisms, primarily on the cellular level. However, the effects of EMR at low-intensity exposure on animals and state of metabolic systems are not fully defined yet. Thus, research of microwave radiation influence on the processes of lipid peroxidation and antioxidant protection system is important for understanding the mechanisms of EMR action on the cell, in particular, and organism development on the whole. The content of lipid peroxidation products – lipid hydroperoxides, thiobarbituric acid reactive substances and the activity of antioxidant enzymes – superoxide dismutase, glutathione peroxidase and catalase in loach embryos under the action of microwave radiation (GSM-900 MHz, SAR = 1.1 Vt/kg) lasting 1; 5; 10 and 20 min during early embryogenesis were studied. It has been found that content of lipid peroxidation products in germ cells undergoes significant changes under the action of low-intensity EMR. The effect of microwave radiation (1, 5, 10 min) leads to the increase of superoxide dismutase activity, nevertheless, 20 min exposure decreased this index to the level of control values as it is shown. It has been established that EMR at frequencies used for mobile communications reduce the activity of antioxidant protection system components, especially catalase and glutathione peroxidase. The growth of catalase activity at the 10-cell stage of blastomere division (P < 0.05) is an exception. The results of two-way analysis of variance attest that microwave radiation factor causes the large part of all observable modifications.
Key words: microwave radiation, loach embryos, lipid peroxidation, superoxide dismutase, catalase, glutathione peroxidase.
The original article in Ukrainian is available for download in PDF format.
© The Ukrainian Biochemical Journal
Journal archive > 2014 > N 5 September-October
I. L. Ryzhko, N. V. Motruk, S. A. Petrov
The influence of salts of heavy metals on trypsin-like peptide hydrolase of drosophila larvae partly refined by methods of salting-out, gel chromatography and electrophoresis has been researched. It is established that cadmium chloride is characterized by the greatest inhibitory effect, while zinc chloride by the lowest one. Since metal chlorides were used in all cases, it is the differentiated effect of metal ions on manifestations of amidase activity of trypsin-like peptide hydrolase of drosophila larvae, which rather may be considered as proved than the effect of chlorine ions. This, as a whole, agrees with the effect of these ions on proteolytic digestion system at the level of live organisms.
Key words: heavy metals, peptide hydrolyse, drosophila.
The original article in Ukrainian is available for download in PDF format.
© The Ukrainian Biochemical Journal