title en

Journal archive > 2013 > N 6 November-December

CHROMOSOMOCENTRIC APPROACH TO OVERCOMING DIFFICULTIES IN IMPLEMENTATION
OF INTERNATIONAL PROJECT HUMAN PROTEOME

A. I. Archakov1, V. G. Zgoda1, A. T. Kopylov1, S. N. Naryzhny1,2, A. L. Chernobrovkin1, E. A. Ponomarenko1, A. V. Lisitsa1

1V. N. Orekhovich Scientific-Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences;
2Petersburg Nuclear Physics Institute at National
Research Centre Kurchatov Institute

The international project Human Proteome (PHP), being a logical continuation of the project Human Genome, was started on September 23, 2010. In correspondence with the genocentric approach, the PHP aim is to prepare a catalogue of all human proteins and to decipher a network of their interactions. The PHP implementation difficulties arise because the research subject itself – proteome – is much more complicated than genome. The major problem is the insufficient sensitivity of proteome methods that does not allow detecting low- and ultralow-copy proteins. Bad reproducibility of proteome methods and the lack of so-called «gold standard» is the second major complicacy in PHP implementation. The third problem is the dynamic character of proteome, its instabili­ty in time. The paper deals with possible variants of overcoming these complicacies, preventing from successful implementation of PHP.

Key words: project Human Genome, project Human Proteome, sensitivity limit, mass-spectrometric method of monitoring of multiple responses.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal