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Journal archive > 2013 > N 5 September-October

OBTAINING AND STUDY OF PROPERTIES OF NEW MONOCLONAL ANTIBODIES AGAINST HUMAN IgЕ

O. Yu. Galkin1,2, A. A. Savchenko1, K. I. Nikitina1, O. M. Dugan1

1National Technical University of Ukraine «Kyiv Polytechnic Institute»;
2Hema Ltd., Kyiv, Ukraine;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The original set of 12 clones of hybridomas, producers of monoclonal antibodies (mAbs) against human IgЕ, has been obtained. The study of biological properties of antibodies has been conducted: their specificity, affinity constant and titer in a cultural medium have been established. The obtained mAbs are directed to the two epitope regions on IgE molecule. The first group of mAbs relates to epitope region, represented by three epitopes (two of them overlap and one that does not overlap with others). The second epitope region is represented by only one epitope.

Key words: monoclonal antibodies, hybridomas, human IgE, affinity, epitope mapping.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal