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Journal archive > 2013 > N 1 January-February

THE EFFECT OF POTENTIAL-DEPENDENT POTASSIUM UPTAKE
ON MEMBRANE POTENTIAL IN RAT BRAIN MITOCHONDRIA

O. V. Akopova, V. I. Nosar, L. I. Kolchinskaya, I. N. Mankovska, M. K. Malysheva, V. F. Sagach

Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
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The effect of potential-dependent potassium uptake on the transmembrane potential difference (??m) in rat brain mitochondria has been studied­. It was shown that in potassium concentration range of 0-120 mM the potential-dependent K+-uptake into matrix leads to the increase in respiration rate and mitochondrial depolarization. ATP-dependent potassium channel (K+ATP-channel) blockers, gliben­clamide and 5-hydroxydecanoate, block ~35% of potential-dependent potassium uptake in the brain mitochondria. It was shown that K+ATP-channel blockage results in membrane repolarization by ~20% of control, which is consistent with experimental dependence of ??m on the rate of potential-dependent potassium uptake. Obtained experimental data give the evidence that functional activity of K+ATP-channel is physiologically important in the regulation of membrane potential and energy-dependent processes in brain mitochondria.

Key words: K+, transport, K+ATP-channel, membrane potential, oxygen consumption, brain mitochondria.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal