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Journal archive > 2013 > N 1 January-February

MEMBRANOTHROPIC PROPERTIES OF THE UROCANIC ACID

N. A. Kasian1, O. V. Vashchenko1, L. N. Zavora1, D. S. Sophronov2, L. N. Lisetski1

1Institute for Scintillation Materials, National Academy of Sciences of Ukraine, Kharkiv;
2STC «Institute for Single Crystals», National Academy of Sciences of Ukraine, Kharkiv;
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The effects of urocanic acid (UA) on thermodynamic parameters of model dipalmitoylphosphatidylcholine (DPPC) lipid membrane have been studied by means of  differential scanning calorimet­ry (DSC). The observed ordering effect of UA on the lipid bilayer is reflected in the increase in both the main phase transition temperature and cooperative unit size of the lipid membrane. Analysis of FTIR spectra suggests localization of UA molecules in the vicinity of the polar heads and carbonyl groups of  DPPC due to electrostatic interactions and H-bonds. On the basis of experimental data obtained and geometry parameters of UA and DPPC molecules, some variants of the UA localization in DPPC bilayer were discussed.

Key words: urocanic acid, multilamellar membrane, dipalmitoylphosphatidylcholine, differential scanning calorimetry, FTIR spectroscopy.

The original article in Russian is available for download in PDF format.

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