COMPARATIVE INVESTIGATION OF THE EFFECT OF CALIX[4]ARENE C-99 AND ITS ANALOGS ON Nа+,K+-ATPase ACTIVITY OF UTERUS MYOCITE PLASMA MEMBRANE (T.O. Veklich, A.A. Shkrabak, S.O. Cherenok, V.I. Kalchenko, S.O. Kosterin)

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Journal archive > 2012 > N 6 November-December

COMPARATIVE INVESTIGATION OF THE EFFECT OF CALIX[4]ARENE C-99 AND ITS ANALOGS

ON Nа⁺,K⁺-ATPase ACTIVITY OF UTERUS MYOCITE PLASMA MEMBRANE

T. O. Veklich¹, A. A. Shkrabak¹, S. O. Cherenok², V. I. Kalchenko², S. O. Kosterin¹

¹Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;

²Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;

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The aim of our investigation was to determine structural features of calix[4]arene C-99 which are important for its inhibition properties relative to Nа⁺,K⁺-ATPase of uterus myocite plasma membrane. Therefore we studied the effect of calix[4]-arenes С-296, С-297, С-424, С-425, С-426, С-427, which are structurally similar to this inhibitor, on the mentioned enzyme activity. We have shown that calixarenes С-296 and С-297 which have two additional propoxy groups on the lower rim of macrocycle are less effective inhibitors of Nа⁺,K⁺-ATPase relative to calixarene C-99. Calixarenes С-425 and С-427 which have on the upper rim of macrocycle three and four phosponic residues, respectively, also inhibit Nа⁺,K⁺-ATPase activity less effectively as compared to calixarene C-99. Both calixarenes: С-424, which has only two carbonate residues on the upper rim, and С-426, which has on the upper rim ketomethilphosphonate residues instead of hydroxymethilphosphonate residues of calixarene C-99, do not affect Nа⁺,K⁺-ATPase activity. We have made respective conclusions concerning the role of certain chemical groups of calixarene C-99 during its interaction with Nа⁺,K⁺-ATPase.

Key words: Na⁺,K⁺-ATPase, Mg²⁺-ATPase, plasma membrane, smooth muscle cells, myometrium, kinetic properties of ATPase, calixarenes, aminophosphonic acids.

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