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Journal archive > 2012 > N 6 November-December

COMPARATIVE INVESTIGATION OF THE EFFECT OF CALIX[4]ARENE C-99 AND ITS ANALOGS
ON Nа+,K+-ATPase ACTIVITY OF UTERUS MYOCITE PLASMA MEMBRANE

T. O. Veklich1, A. A. Shkrabak1, S. O. Cherenok2, V. I. Kalchenko2, S. O. Kosterin1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;
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The aim of our investigation was to determine structural features of calix[4]arene C-99 which are important for its inhibition properties relative to Nа+,K+-ATPase of uterus myocite plasma membrane. Therefore we studied the effect of calix[4]-arenes С-296, С-297, С-424, С-425, С-426, С-427, which are structurally similar to this inhibitor, on the mentioned enzyme activity. We have shown that calixarenes С-296 and С-297 which have two additional propoxy groups on the lower rim of macrocycle are less effective inhibitors of Nа+,K+-ATPase relative to calixa­rene C-99. Calixarenes С-425 and С-427 which have on the upper rim of macrocycle three and four phosponic residues, respectively, also inhibit Nа+,K+-ATPase activity less effectively as compared to calixarene C-99. Both calixarenes: С-424, which has only two carbonate residues on the upper rim, and С-426, which has on the upper rim ketomethilphosphonate residues instead of hydro­xymethilphosphonate residues of calixarene C-99, do not affect Nа+,K+-ATPase activity. We have made respective conclusions concerning the role of certain chemical groups of calixarene C-99 during­ its interaction with Nа+,K+-ATPase.

Key words: Na+,K+-ATPase, Mg2+-ATPase­, plasma membrane, smooth muscle cells, myometrium, kinetic properties of ATPase, calixarenes, aminophosphonic acids.

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