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Journal archive > 2012 > N 6 November-December

GENERATION OF SUPEROXIDE ANION-RADICAL IN THE LIVER MONOOXYGENASE
SYSTEM OF PRELIMINARY RADIATION-EXPOSED TUMOR-BEARING RATS

М. М. Marchenko, O. V. Ketsa

Yuriy Fedkovych Chernivtsi National University, Ukraine;

Generation of superoxide anion-radical (O2•-) in reductase and oxygenase electron-transport chains in the liver monooxygenase system was investigated in tumor-bearing rats exposed to preliminary irradiation. Preliminary irradiation of rats (before transplantation of Guerin’s carcinoma) resulted in the increased generation of superoxide anion-radical by components of the liver monooxygenase system in the logarithmic phase of oncogenesis­. It is shown that the increased NADPH-cytochrome P-450 reductase activity is accompanied with the intensification of superoxide anion-radical generation in liver microsomal fraction of preliminary radiation-exposed rats. At the same time the cytochrome P-450 hydroxylase activity in the microsomal fraction of tumor-bearing rats subjected to preliminary irradiation was decreased.

Key words: superoxide anion-radical, NADPH-cytochrome P-450 reductase, cytochrome P-450, monooxygenase system, microsomal fraction, liver, Guerin’s carcinoma.

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© The Ukrainian Biochemical Journal