А. K. Gulevsky, A. Yu. Nikolchenko
The influence of the penetrating cryoprotector and Mg2+ ions on the protein-synthesizing activity of postmitochondrial supernantant of the rat liver as well as on aminoacylation processes has been investigated. The addition of the penetrating cryoprotectors – ethylene glycol and DMSO – resulted in the concentration-dependant reversible inhibition of the protein biosynthesis and aminoacylation reaction in the cell-free system. These cryoprotectors at low concentrations intensified the stimulating effect of Mg2+ on the cumulative protein synthesis in the cell-free system.
Key words: protein synthesis in the cell-free system, aminoacylation, penetrating cryoprotectant, ions Mg2+.
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