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Journal archive > 2012 > N 4 July-August

INFLUENCE OF CHEMICALLY DIFFERENT ANTIVIRAL SUBSTANCES ON THE EXPRESSION
OF IFN?, PKR, OAS1a AND RNAse L GENES

О. O. Dragushchenko1,2, I. I. Minia1, T. A. Poliezhaeva1,2, D. B. Starosyla3, I. S. Karpova1, M. Yu. Obolenska1, S. L. Rybalko3

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Taras Shevchenko Kyiv National University, Institute of Biology, Ukraine;
3State Institution L. V. Gromashevskiy Institute of Epidemiology and Infectious Diseases,
National Academy of Medical Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The aim of the study was to determine the ability of several chemically different antiviral substances to induce the expression of interferon ? (IFN?), PKR, OAS1a and RNAse L genes in the rat liver. The investigated substances included Amizon, Altabor and Proteflasid, which are already used in practical medicine, and 3?,7-dimethylquercetin extracted from Proteflasid, the mixture of synthesized trimethyl- and tetramethylquercetin and Sialospecific lectin from persimmon, which are at the stage of preclinical trial and experimental research respectively.

The content of corresponding mRNAs in total RNA was detected with the help of reverse transcription and polymerase chain reaction in real time. The results have shown that all investigated substances induce the expression of genes IFN?, PKR, OAS и RNAse L in specific manner. The combination of 3?,7-dimethylquercetin + lectin from persimmon had the highest stimulating effect exceeding the effect of each component of the mixture and the influence of Heberon (recombinant IFN?2b) and PolyI-polyC as the standard inducers of IFN? and its target genes.

The ability of all substances to specifically induce the expression of IFN? and its target genes, the absence of correlation between the levels of IFN? and its target genes expression as well as between target genes themselves indicate that the mechanism of antiviral activity of the investigated substances is connected not only with up-regulation of IFN? and potential IFN? mediated effects.

Key words: antiviral medicines, Amizon, Altabor, Proteflasid, derivatives of quercetin, lectin from persimmon, interferon ?, target genes.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal