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Journal archive > 2012 > N 3 May-June

THE INFLUENCE OF N-STEAROYLETHANOLAMINE ON THE ACTIVITY OF ANTIOXIDANT ENZYMES
AND ON THE LEVEL OF STABLE NO METABOLITES IN THE RAT TESTES AND BLOOD PLASMA
AT THE EARLY STAGES OF STREPTOZOTOCINE-ІNDUCED DIABETES

T. M. Goridko, G. V. Kosiakova, A. G. Berdyschev, V. R. Bazylyanska, V. M. Margitich, N. M. Gula

Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;

The influence of N-stearoylethanolamine was investigated on the activity of enzymes of antioxidant protection and content of stable metabolites of nitric oxide (NO) in the testes and plasma of rats at the early stages of development of streptozotocine-induced diabetes mellitus. It was shown that the activity of superoxide dismutase, catalase is reduced in the plasma and testes of animals with streptozotocin-induced (50 mg/kg) diabetes (blood glucose 8-10 mmol/L). A significant increase in the amount of nitrite and nitrate anions  was revealed in the plasma of rats, while only the level of nitrite was significantly changed in the testes of animals.  The per os administration of the NSE aqueous suspension in a dose of 50 mg/kg during 10 days to the rats with induced diabetes contributed to the normalization of catalase activity in the testis, which correlated with a decrease in the amount of TBA-reacting products and activity of superoxide dismutase and catalase in the blood plasma of animals; the use of NSE also contributed to the reduction of nitrite content in the gonads and to normalization of both nitrite and nitrate in the blood plasma of rats. The NSE administration to intact animals caused an increase in superoxide dismutase activity and significantly reduced the content of stable NO metabolites in the blood plasma of animals.

Key words: N-stearoylethanolamine, experimental diabetes mellitus (EDM), enzymes of antioxidant protection, NO, POL, testes.

The original article in Ukrainian is available for download in PDF format.

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