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Journal archive > 2012 > N 3 May-June

EFFECT OF SODIUM CHLORIDE AND NITROPRUSSIDE ON PROTEIN CARBONYL GROUPS
CONTENT AND ANTIOXIDANT ENZYME ACTIVITY IN LEAVES OF CORN SEEDLINGS Zea mays L.

Yu. V. Vasylyk1, N. M. Semchuk1, Ok. V. Lushchak2, V. I. Lushchak1

1Department of Biochemistry and Biotechnology,
2Botanical Gardens of Vasyl Stefanyk Precarpathian National University,
Ivano-Frankivsk, Ukraine;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The effect of sodium nitroprusside (SNP) and sodium chloride (NaCl) on protein carbonyl group content and activity of antioxidant enzymes was investigated in leaves of maize seedlings. Incubation with NaCl and SNP+NaCl increased the content of carbonyl proteins after 24 h. Treatment with SNP+NaCl during 48 h showed lower and after 72 h higher carbonyl protein content than that in the control. Catalase activity was higher in the leaves of SNP+NaCl-treated than in the leaves of SNP-treated seedlings after 24 h. Ascorbate peroxidase activi­ty increased after incubation with 0.2 mM SNP for 24 h. Significant increment of guaiacol peroxidase activity was obtained in all treated groups in comparison with the control after 72 h. Glutathione-S-transferase activi­ty increased after 48 h seedling treatment with NaCl or SNP and 72 h seedling incubation with NaCl. Under experimental conditions used, glutathione reductase activity was virtually not affected. It is proposed that SNP can be used to prevent salt-induced oxidative stress in maize.

Key words: corn seedlings, salt stress, sodium nitroprusside, oxidative stress, antioxidant enzymes.

The original article in English is available for download in PDF format.

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