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Journal archive > 2012 > N 1 January-February

GENERATION OF ACTIVE OXYGEN FORMS IN RAT TYMOCYTES
UNDER ACTION OF HYDROGEN PEROXIDE AND FULLERENE C60

S. M. Grebinyk, I. I. Grynyuk, S. V. Prylutska, O. P. Matyshevska

Taras Shevchenko Kyiv National University, Ukraine;

The dynamics of active oxygen forms (AOF) generation in rat thymocytes 50 min after treatment with 0.1 and 0.5 mM H2O2 was estimated with the use of fluorescent probe DCFDA. Both enhanced AOF generation, which was dependent on H2O2 concentration, and glutathione peroxidase and superoxide dismutase activation, followed by a decrease of thymocytes viability were demonstrated. Preincubation of cells with 10-5 M fullerene C60 was shown not only to prevent H2O2 – induced AOF generation but to increase viability of H2O2-treated thymocytes at more prolonged time period. The data obtained indicate to fullerene C60 ability to prevent oxidative stress in thymocytes.

Key words: thymocytes, DCF, hydrogen peroxide, AOF, fullerene C60, cell viability, antioxidant enzymes.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal