Journal archive > 2012 > N 1 January-February

INVESTIGATION OF TOMATO (Solanum lycopersicum) AMINOTRANSFERASES
INVOLVED IN BIOSYNTHESIS OF BRANCHED-CHAIN-AMINO-ACIDS

 

A. S. Kochevenko1,2, A. R. Fernie2

1Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv;
2Max Planck Institute of Molecular Plant Physiology, Golm, Germany;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

This study presents evidence for the role of ВСАТ3 and ВСАТ4 proteins in the synthesis of branched-chain-amino-acids in tomato Solanum lycopersicum. ВСАТ3 and ВСАТ4 genes were located­ on tomato chromosomal map by RFLP method (restriction fragment length polymorphism). Using confocal microscopy it was shown that BCAT3-GFP and BCAT4-GFP fusion proteins were localised in chloroplasts. It was observed that these aminotransferase isoforms exhibited distinct kinetic properties and a differential expression pattern of mRNA levels in various tomato tissues.

Key words: Solanum lycopersicum, branched-chain-amino-acids, aminotransferases, green fluorescent protein, recombinant protein.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal