This study presents evidence for the role of ВСАТ3 and ВСАТ4 proteins in the synthesis of branched-chain-amino-acids in tomato Solanum lycopersicum. ВСАТ3 and ВСАТ4 genes were located on tomato chromosomal map by RFLP method (restriction fragment length polymorphism). Using confocal microscopy it was shown that BCAT3-GFP and BCAT4-GFP fusion proteins were localised in chloroplasts. It was observed that these aminotransferase isoforms exhibited distinct kinetic properties and a differential expression pattern of mRNA levels in various tomato tissues.
Key words: Solanum lycopersicum, branched-chain-amino-acids, aminotransferases, green fluorescent protein, recombinant protein.
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