Journal archive > 2011 > N 6 November-December

METHOD FOR ENDOGENOUS FORMALDEHYDE EVALUATION IN ANIMAL ORGANISM

S. G. Shandrenko, M. M. Savchyk, M. P. Dmytrenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

A method for endogenous formaldehyde (FA) level evaluation has been worked out. The method involves the administration of dimedone, which forms the stable complex with FA, and the determination of formaldimedone concentration in biological samples by the fluorescence approach. The method was tested on rat’s models of FA metabolism modulation. Animals received FA (10 mg/kg); or methylamine - substrate of FA-generating enzyme SSAO, (250 mg/kg); or semicarbazide - SSAO inhibitor, (200 mg/kg). Concentration of FA bound with dimedone in the liver tissue were, correspondingly: 7.5 ± 1.5 mkg/kg; 5.4 ± 0.9 mkg/kg; 2.4 ± 0.7 mkg/kg; control – 4.2 ± 1.4 mkg/kg. Obtained data indicate, that the elaborated method­ gives reliable information about FA level.

Key words: formaldehyde, dimedone, formaldimedone, methylamine, semicarbazide.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal