INFLUENCE OF VARIUOS REGIMENS ULTRASAUND
ON OXIDE-MODIFIED ACTOMYOSIN SUPERPRECIPITATION
REACTION FROM SKELETAL MUSCLE OF RABBIT
Taras Shevchenko Kyiv National University, Ukraine;
A comparative study of rabbit skeletal muscles oxide-modified actomyosin superprecipitation reaction in dependence on continuous and impulsive (2 ms) ultrasound regimens was studied. From the analyses of kinetic curves the effect of the value of superprecipitation (Аm – А0), time t1/2, required for achievement of half of its value was determined, and the normalized maximal rate of this reaction Vn was also calculated. It is shown that the use of continuous ultrasound to oxide-modified actomyosin was associated with a significant decrease of superprecipitation relative to controls. However, pulsed ultrasound caused a significant increase in superprecipitation value except for the values (Аm – А0) in the application of the intensity of 0.2 W/cm2. The oxide-modified actomyosin superprecipitation value under the effect of continuous and impulsive ultrasound at intensity 1 W/cm2 in relative to control and all other applied intensities decrease to the most extent. It is caused perhaps by thermal influence of ultrasound. In general, the data obtained give reason to assume that the effects of continuous and pulsed ultrasound on the reaction of oxide-modified proteins complex superpretsipitatsii identical.
Key words: ultrasound, actomyosin, oxidative modification, superprecipitation, kinetic parameters.
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