Journal archive > 2011 > N 6 November-December

FUNCTION OF TRANSPORT H+-ATPases IN PLANT CELL PLASMA AND VACUOLAR
MEMBRANES OF MAIZE UNDER SALT STRESS CONDITIONS AND EFFECT
OF ADAPTOGENIC PREPARATIONS

Zh. I. Ribchenko, Т. A. Palladina

Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv;
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Participations of electrogenic H+-pumps of  plasma and vacuolar membranes represented by E1-E2 and V-type H+-ATPases in plant cell adaptation to salt stress conditions has been studied by determination of their transport activities. Experiments were carried out on corn seedlings exposed during 1 or 10 days at 0.1 M NaCl. Preparations Methyure and Ivine were used by seed soaking at 10-7 M. Plasma and vacuolar membrane fractions were isolated from corn seedling roots. In variants­ without NaCl a hydrolytical activity of plasma membrane H+-ATPase was increased with seedling age and its transport one was changed insignificantly, wherease the response of the weaker vacuo­lar H+-ATPase was opposite. NaCl exposition decreased  hydrolytical activities of both H+-ATPases and increased their transport ones. These results demonstrated amplification of H+-pumps function especially represented by vacuolar H+-ATPase. Both preparations, Methyure mainly, caused a further increase of transport activity which was more expressed in NaCl variants. Obtained results showed the important role of these H+-pumps in plant adaptation under salt stress conditions reali­zed by energetical maintenance of the secondary active Na+/H+-antiporters which remove Na+ from cytoplasm.

Key words: salinity, transport and hydrolytic activity of H+-ATPase, vacuolar, Methyure, Ivine.

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