COMPARATIVE INVESTIGATION BY SPECTROFLUORIMETRY AND FLOW CYTOMETRY OF PLASMA AND INNER
MITOCHONDRIAL MEMBRANES POLARISATION IN SMOOTH MUSCLE CELL USING POTENTIAL-SENSITIVE PROBE DIOC6(3)
Palladine Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
Possibility of the use of flow cytometry and spectrofluorimetry analysis for investigation mitochondria and plasma membrane polarization in myometrium cell suspension using potential-sensitive probe 3,3?-dihexyloxacarbocyanine [DiOC6(3)] has been demonstrated. The obtained results confirm the use of DiOC6(3) for studying the influence of effectors on transmembrane potentials of intact cell compartments.
Key words: transmembrane potential, potential-sensitive probe, flow cytometry, myometrium cell.
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