Journal archive > 2011 > N 4 July-August

RNA INTERFERENCE OF PROTEASOME SUBUNIT OF PSM?7 GENE RESTRICTS
PROTEASOME SUBUNIT PSM?1 AND PSM?5 mRNA EXPRESSION AND PEPTIDYL-GLUTAMYL
PEPTIDE-HYDROLYZING PROTEASOME ACTIVITY IN NEONATAL CARDIOMYOCYTES

V. O. Kyrychenko, D. O. Pashevin, L. V. Tumanovska, V. E. Dosenko, O. O. Moibenko

Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
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Using small interfering RNA (siRNA) transfection of neonatal cardiomyocytes to inhibit expression of nonproteolytic proteasome ?7 subunit, we observed a significant decrease in ?1 proteolytic subunit mRNA expression. Proteasome peptidyl-glutamyl peptide-hydrolyzing activity decreased to 28% (0.48 ± 0.2 nM AMC/min) compared to control (1.7 ± 0.5 nM AMC/min) (Р < 0.05). ?5 Subunit mRNA expression decreased 21 times (Р < 0.05) with no changes in its chymotrypsin-like activity. Proteasome trypsin-like activity and activity of another proteolytic enzyme tripeptidylpeptidase II remained unchanged.

Key words: proteasome, proteasome subunits, RNA-interference, cardiomyocytes.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal