RNA INTERFERENCE OF PROTEASOME SUBUNIT OF PSM?7 GENE RESTRICTS
PROTEASOME SUBUNIT PSM?1 AND PSM?5 mRNA EXPRESSION AND PEPTIDYL-GLUTAMYL
PEPTIDE-HYDROLYZING PROTEASOME ACTIVITY IN NEONATAL CARDIOMYOCYTES
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
Using small interfering RNA (siRNA) transfection of neonatal cardiomyocytes to inhibit expression of nonproteolytic proteasome ?7 subunit, we observed a significant decrease in ?1 proteolytic subunit mRNA expression. Proteasome peptidyl-glutamyl peptide-hydrolyzing activity decreased to 28% (0.48 ± 0.2 nM AMC/min) compared to control (1.7 ± 0.5 nM AMC/min) (Р < 0.05). ?5 Subunit mRNA expression decreased 21 times (Р < 0.05) with no changes in its chymotrypsin-like activity. Proteasome trypsin-like activity and activity of another proteolytic enzyme tripeptidylpeptidase II remained unchanged.
Key words: proteasome, proteasome subunits, RNA-interference, cardiomyocytes.
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