Journal archive > 2011 > N 1 January-February

THE CALIXARENE C-107 INCREASES THE AFFINITY OF THE Na+,K+-АТРase ACTIVITY
IN PLASMATIC MEMBRANE OF SMOOTH MUSCLE CELLS TO THE OUABAIN

T. O. Veklich1, A. A. Shkrabak1, R. V. Rodik2, V. I. Kalchenko2, S. O. Kosterin1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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2Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;
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In the experiments carried out with the suspension of the myometrium cell plasmatic membranes treated with 0.1% digitonin solution we investigated the influence of сalixarene С-107 (5,17-diamino(2-pyridyl)methylphosphono-11,23-di-tret-butyl-26,28-dihydroxy-25,27-dipropoxycalix[4]arene) on the Nа+,K+-АТРase activity. It was shown that this calixarene increased the affinity of the enzyme for the sodium pump conventional inhibitor - ouabain: the magnitudes of the seeming­ constant of inhibition I0.5changed from 26.9 ± 1.3 mM to 10.9 ± 0.6 mM. However the ouabain itself did not influence on the affinity of the Nа+,K+-АТРase for сalixarene С-107.

Key words: Na+,K+-ATPase, Mg2+-ATP­ase, plasma membrane, smooth muscle cells, myometrium, enzymatic hydrolysis of ATP, kinetic properties of ATPase, calixarenes, aminophosphonic acids.

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