THERMAL INACTIVATION OF Penicillium canescens ?-GALACTOSIDASE
Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv;
The kinetics and mechanism of thermal inactivation of Penicillium canescens ?-galactosidase in the temperature range of 55-65 °C have been studied. The kinetic scheme of ?-galactosidase thermal inactivation was proposed which included the reversible dissociation of active hexamers into associating monomers and irreversible denaturation of monomers. The kinetic constants of thermal inactivation have been determined. The effect of enzyme concentration and purification efficiency has been investigated. A possibility of defence of protein molecule from thermal inactivation in the presence of BSA, glycerol, melibiose, raffinose and stachyose is shown.
Key words: ?-galactosidase, Penicillium canescens, thermal inactivation, constants of thermal inactivation.
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