relationship between the reaching of equilibrium and the accuracy
of the affinity determination for antibody-antigen reaction
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
Theoretical investigation of the relationship between the reaching of equilibrium and the accuracy of the affinity determination for antibody-antigen reaction were carried out. It was shown that evaluation of the reagent concentrations before reaching the dynamic equilibrium leads to a decrease of estimation accuracy for the affinity constant of this reaction. We have established that for most of typical antibodies the dynamic equilibrium of their reaction with corresponding antigens comes 2-4 hours after the beginning.
Key words: antigen-antibody reaction, affinity, dynamic equilibrium.
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