Journal archive  2010  #4 July-August

PHOSPHOLIPIDS COMPOSITION OF NORMAL AND TRANSFORMED CELLS
CULTIVATED WITH N-ACYLETHANOLAMINES

V. S. Asmolkova, G. I. Lavrenchuk, V. M. Klimashevskyi, N. M. Gula

Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
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Effects of N-stearoylethanolamine (NSE), N-oleoilethanolamine (OEA) and mixture of more than 20 saturated and unsaturated N-acylethanolamines on phospholipids composition of normal and transformed fibroblasts in the culture were compared in the work.
It was shown that cultivation of cells NSE decreases percent content of phosphatidylinositol (PI), phosphatidylserine (PS) and  sphingomyeline (SM) – important mediators of cell signaling. Under the influence of ОЕА the level of SM decreases as well, but at the same time PI and PS levels increase. NAEs mixture decreases PS and PI levels in cells and causes phosphatidylcholine (PC) amount increase. Moreover NSE and NAEs mixture decrease the content of main mitochondria membrane lipid – diphosphatidylglicerole (DPG). ОЕА increases DPG amount.
In transformed fibroblasts (line L929) NAE modulate lipid composition as well: NSE decreases the level of phosphatidylethanolamine (PE), ОЕА and NAEs mixture increase sphingomyeline levels.
It is shown that response of normal and transformed fibroblasts on NAE application is different, depending on substance structure and cell-target type.

Key words: N-acylethanolamines, phospholipids, fibroblasts, transformed cells, cell culture.

The original article in Ukrainian is available for download in PDF format.