Journal archive   2010  #4 July-August

FIBRONECTIN-1 EXPRESSION IN LYMPHOCYTES OF PATIENTS WITH ERYTHREMIA DISEASE

A. O. Kulinich1, D. O. Minchenko2, A. S. Maslak1,
A. I. Shevtsova1, O. Z. Brazaluk1, O. H. Minchenko2

1Dnipropetrovsk State Medical Academy, Ukraine;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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Expression of fibronectin-1 mRNA in lymphocytes in erythremia patients and healthy donors as well as fibronectin-1 concentration in plasma and its heparin-binding activity have been studied. Moreover, we also investigated the expression of fibronectin protein in lymphocytes and cell surface in erythremia disease as compared to healthy donors. It was shown that fibronectin-1 mRNA expression in lymphocytes is increased in patients with erythremia as compared to healthy donors. The decrease of plasma fibronectin concentration and its heparin-binding activity as well as the increase of lymphocyte content with surface-associated and intracellular fibronectin were revealed in erythremia disease in comparison with healthy donors. Positive correlation between plasma fibronectin level and its heparin-binding activity and negative correlation between plasma fibronectin level and quantity of lymphocytes which express fibronectin inside the cell and on cell surface was detected. Results of this investigation demonstrate that fibronectin-1 mRNA expression in lymphocytes is disturbed in erythremia disease and is accompanied by a decrease of fibronectin plasma level.

Key words: fibronectin-1, mRNA expression, lymphocyte, plasma,  erythremia.

The original article in Ukrainian is available for download in PDF format.