COMPARISON OF NO PRODUCTION LEVEL AND BREAST ADENOCARCINOMA CELLS MCF-7
PROLIFERATION UNDER MODIFICATION CONDITION OF MICROENVIRONMENT
1Department of Biotechnical Problems of Diagnostic Institute of Problems
of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kyiv;
2Taras Shevchenko Kyiv National University, Ukraine
Connection between the level of NO generation and its cytotoxic influence was investigated on two models of tumor cells (breast adenocarcinoma MCF-7) culture: monolayer and spheroids. Conditions of microenvironment were modified by Са2+ channels blockers: EGTA, nitrendipine and Cl-lanthan. It was discovered in the work that deviation in concentration of extracellular Са2+ under the influence of EGTA leads to reducing of the level of extracellular NO to 50% (Р ? 0.05) of control and increasing of the number of live cells by 10-40% (Р ? 0.05). Nitrendipin has the properties selective Са2+ channels blocker that reduced the level of NO by 35-70% (Р ? 0.05), depending on the substances concentration and time of incubation. At that time proliferation potential of cells increased by 10-35% (Р ? 0.05). NO production in MCF-7 cells under the influence of Cl-lanthan in concentration of 0.01 and 0.1 mM is reduced by 50-60%, as compared with the control. But it had no influence on the number of live cells. On 2-D and 3-D models of tumor growth it was demonstrated that extracellular NO production in monolayer of MCF-7 was 3.2 pmol for 106 cells and for spheroids it was 8.5 pmol.
Key words: nitrogen oxide, NO-synthase, 2-D and 3-D models of tumor growth, Са2+ channels blockers.
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