Journal archive   2010  #4 July-August

COMPARISON OF NO PRODUCTION LEVEL AND BREAST ADENOCARCINOMA CELLS MCF-7
PROLIFERATION UNDER MODIFICATION CONDITION OF MICROENVIRONMENT

O. M. Perepelytsina1, L. V. Garmanchouk2, E. A. Shevchenko2,
M. V. Sydorenko1, L. I. Ostapchenko2

1Department of Biotechnical Problems of Diagnostic Institute of Problems
of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.;
2Taras Shevchenko Kyiv National University, Ukraine

Connection between the level of NO generation and its cytotoxic influence was investigated on two models of tumor cells (breast adenocarcinoma MCF-7) culture: monolayer and spheroids. Conditions of microenvironment were modified by Са2+ channels blockers: EGTA, nitrendipine and Cl-lanthan. It was discovered in the work that deviation in concentration of extracellular Са2+ under the influence of EGTA leads to reducing of the level of extracellular NO to 50% (Р ? 0.05) of control and increasing of the number of live cells by 10-40% (Р ? 0.05). Nitrendipin has the properties selective Са2+ channels blocker that reduced the level of NO by 35-70% (Р ? 0.05), depending on the substances concentration and time of incubation. At that time proliferation potential of cells increased by 10-35% (Р ? 0.05). NO production in MCF-7 cells under the influence of Cl-lanthan in concentration of 0.01 and 0.1 mM is reduced by 50-60%, as compared with the control. But it had no influence on the number of live cells. On 2-D and 3-D models of tumor growth it was demonstrated that extracellular NO production in monolayer of MCF-7 was 3.2 pmol for 106 cells and for spheroids it was 8.5 pmol.

Key words: nitrogen oxide, NO-synthase, 2-D and 3-D models of tumor growth, Са2+ channels blockers.

The original article in Ukrainian is available for download in PDF format.