Journal archive   2010  #4 July-August

STATE OF THE LIVER ANTIOXIDANT SYSTEM AND CONTENT OF MATRIX
METALLOPROTEINASE-2 OF LARGE INTESTINE UNDER THE EFFECT OF MALEIMIDE
DERIVATIVE IN EXPERIMENTAL COLON CARCINOGENESIS IN RATS

O. M. Filinska, S. V. Yablonska, S. Y. Mandryk, I. V. Kharchuk,
G. V. Ostrovska, V. K. Rybalchenko

Taras Shevchenko Kyiv National University, Ukraine;
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The maleimide derivative – 1-(4-Cl-benzyl)-3-Cl-4-(CF3-phenylamino)-1H-pyrrol-2.5-dione (MI-1) with cytostatic activity did not cause substantial changes of liver antioxidant system and level of matrix metalloproteinase-2 in intestinal mucosa after chronic treatment (for 20 weeks). MI-1 did not cause significant changes in the content of thiobarbituric-active products and plasma membrane protein carbonyl groups in the rat liver. However activities of superoxide dismutase, glutathione peroxidase, and content of reduced glutathione were decreased in both doses – 0.027 and 2.7 mg/kg. The level of matrix metalloproteinase-2 in intestinal mucosa was decreased just in maximum dose – 2.7 mg/kg. The contents of thiobarbituric-active products, protein carbonyl groups, reduced glutathione, matrix metalloproteinase-2, activities of glutathione peroxidase and glutathione-S-transferase in the liver cells have increased in 1.2-dimethylhydrazine-induced colon cancer in rats. The activities of enzymes of the first line of antioxidant defense – superoxide dismutase and catalase were decreased to 40%. The maleimide derivative prevents development of oxidation  stress and partially reduce them to control level.

Key words: antioxidant system, peroxidation, colon carcinogenesis, maleimide derivative, 1.2-dimethylhydrazine.

The original article in Ukrainian is available for download in PDF format.