STATE OF THE LIVER ANTIOXIDANT SYSTEM AND CONTENT OF MATRIX
METALLOPROTEINASE-2 OF LARGE INTESTINE UNDER THE EFFECT OF MALEIMIDE
DERIVATIVE IN EXPERIMENTAL COLON CARCINOGENESIS IN RATS
Taras Shevchenko Kyiv National University, Ukraine;
The maleimide derivative – 1-(4-Cl-benzyl)-3-Cl-4-(CF3-phenylamino)-1H-pyrrol-2.5-dione (MI-1) with cytostatic activity did not cause substantial changes of liver antioxidant system and level of matrix metalloproteinase-2 in intestinal mucosa after chronic treatment (for 20 weeks). MI-1 did not cause significant changes in the content of thiobarbituric-active products and plasma membrane protein carbonyl groups in the rat liver. However activities of superoxide dismutase, glutathione peroxidase, and content of reduced glutathione were decreased in both doses – 0.027 and 2.7 mg/kg. The level of matrix metalloproteinase-2 in intestinal mucosa was decreased just in maximum dose – 2.7 mg/kg. The contents of thiobarbituric-active products, protein carbonyl groups, reduced glutathione, matrix metalloproteinase-2, activities of glutathione peroxidase and glutathione-S-transferase in the liver cells have increased in 1.2-dimethylhydrazine-induced colon cancer in rats. The activities of enzymes of the first line of antioxidant defense – superoxide dismutase and catalase were decreased to 40%. The maleimide derivative prevents development of oxidation stress and partially reduce them to control level.
Key words: antioxidant system, peroxidation, colon carcinogenesis, maleimide derivative, 1.2-dimethylhydrazine.
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