COMPARATIVE STUDY IN THE IN VITRO AND IN VIVO EXPERIMENTS
OF INFLUENCE OF OUABAIN AND CALIXARENE C107 ON Na+,K+-ATPase
ACTIVITY OF RAT HEPATOCYTE PLASMATIC MEMBRANES
1Taras Shevchenko Kyiv National University, Ukraine;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;
The comparative study of influence of ouabain and calixarene C107, and the structure component of this calixarene – fragment M3, in the conditions of in vitro and chronic action in vivo on Na+,K+-ATPase activity was carried out on the fractions of plasmatic membranes (PM) of the rat hepatocytes. A general property in the conditions in vitro is the ability of calixarene C107 and ouabain (both substances were in the concentration of 1 mM) to inhibit PM Na+,K+-ATPase of rat hepatocytes. However, in the case of activities of calixarene C107 and ouabain in the conditions in vivo heterogeneous action on Mg2+-ATP-ase and Mg2+,Na+,K+-ATP-ase activities takes place: total activity in the conditions of injection of increased concentrations of ouabain remains without changes, but Mg2+-ATP-ase activity significantly grows; in analogous conditions under the action of calixarene C107 both these activities decrease twice in comparison with control. Both under the in vitro and in vivo conditions, M3 fragment (the structural component of C107) does not change the values of investigated enzymatic activities. The biochemical mechanisms of calixarene C107 action on Na+,K+-ATP-ase activity in PM of rat hepatocytes are discussed.
Key words: hepatocytes, ouabain, calixarene C107, activity in vitro and in vivo Mg2+,Na+,K+-ATPase, Mg2+-ATPase, Na+,K+-ATPase.
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