THE INFLUENCE OF POTASSIUM 4-TOLUENETHIOSULFONATE ON?MEMBRANE POTENTIAL
AND? ATPase ACTIVITY OF PLASMATIC MEMBRANES OF LOACH EMBRYOS
1Lviv National Polytechnic University, Ukraine;
2Ivan Franko L’viv National University, Ukraine;
The influence of novel biologically active substance potassium 4-toluenethiosulfonate in concentration of 4·10-5 M on the changes of electrophysiological parameters of embryonic cells in early development of fish (Misgurnus fossilis L.) was investigated including the changes of membrane potential (TMP) and enzyme activity of plasmatic membranes of loach embryos during the period of synchronous division of blastomers in the early period of development. The evaluation of influence of these matters was studied and aperiodic changes of the level of TMP were shown. The diminishing of amplitude in every period by 7?12?mV and diminishing of growth of maximal values of vibrations of TMP by 39 mV in comparison to control was noticed. It was related to inhibition of some biosynthetic processes and results in the decline of activity of membrane enzyme (Na+,K+-АТРase) by 75.5 ± 4.1% and 78.4?±?10.4% both at the action of high (4·10-3 М) and low (4·10-9 М) concentrations, accordingly, at first hours of development with subsequent renewal of its activity to the level of control only for the actions of low concentration.
Key words: thiosulfonate, membrane potential, Na+,K+-АТPase, loach embryos, plasma membrane, blastomers divisions, mitochondria, rough endoplasmic reticulum.
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