PROTECTIVE EFFECT OF N-STEAROYLETHANOLAMINE UNDER ACUTE ALCOHOL INTOXICATION IN RATS
N. M. Gula, T. M. Goridko, N.A.Stogniy, V. M. Klymashevsky, O. F. Meged, G.V.Kosyakova, S. A. Shovkun, N.L.Kindruk, A. G. Berdyshev
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
The influence of N-stearoylethanolamine on the nitric oxide system, the state of lipid peroxidation, antioxidant enzyme activity, content of phospholipids and fatty acids were studied under the acute ethanol intoxication (2.5 g/kg) in rats.
The results of investigations show that acute ethanol intoxication caused abnormalities of the oxidative homeostasis accompanied by the accumulation of thiobarbituric acid reactive substances (TBARS). High catalase and glutathione peroxidase activity was also shown. The altered content of total and individual fatty acids of phospholipids in the rat liver tissue was found. The content of saturated fatty acids (palmitic, stearic) increased and amount of unsaturated (palmitoleic, oleic, linolenic) acids decreased under acute ethanol intoxication. The changes of nitric oxide content was found in the brain, plasma and red blood cells. N-stearoylethanolamine (NSE) in a dose of 50?mg/kg of body weight shows the pronounced antioxidative and hepatoprotective properties under these conditions. It was found that the preliminary NSE administration to rats inhibited accumulation of TBARS and caused a simultaneous increase of antioxidant enzyme activity. The NSE administration modulated also the content of total and individual fatty acids of phospholipids and the amount of nitric oxide in pathologically altered tissues. These results suggested that NSE protected the structural integrity and functional ability of cell membranes under the acute ethanol intoxication.
Key words: N-stearoylethanolamine, lipid peroxidation, antioxidative enzymes, nitric oxide, phospholipids, fatty acids, cholesterol, ethanol.
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