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Journal archive > 2010 > N 1 January-February

REGISTRATION OF К+-EQUILIBRIUM POTENTIAL IN MYOMETRIUM CELL PLASMA MEMBRANE
AND STUDY OF ITS MODULATION OF NOX AND Н2О2 USING FLOW CYTOMETRY METHOD

G. V. Danylovych, Yu. V. Danylovych, V.F.Gorchev

Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
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Prospects of the use of flow cytometry analy­sis for investigation of forming К+- equilibrium membrane potential on the experimental model of myometrium plasma membrane vesicles in the presence of valinomycine using potential-sensitive probe of DiOC6(3). Transmembrane potential magnitude corresponds to magnitude by Nernst’s equation.
Н2О2 and NO2-, probably, increase permeability of membrane for К+ and result in potential dissipation. Given effect is not shown for sodium nitroprusside. The obtained results confirm an assumption as to enhancing the passive transport for К+ through sarcolemma under the action of these substances, that can lead to membrane repolarisation and decline of the level of its excitability.

Key words: hydrogen peroxide, nitric oxi­de, flow cytometry, К+-equilibrium membrane potential, sarcolemma.

The original article in Ukrainian is available for download in PDF format.

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