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Journal archive > 2010 > N 1 January-February

INITIAL ANALYSIS OF THE RESULTS OF THE GENOME-WIDE SEARCH FOR THE GENES
OF RESPONSE TO INTERFERON ALPHA

O. O. Dragushchenko, B. T. Tokovenko, M.?Yu. Obolenskaya

Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
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The paper is focused on the primary analysis of the potential genes of primary response to IFN?, predicted by the program COTRASIF elaborated in our laboratory. Two web-instruments FatiGO and GOTM were applied for this purpose; the literature search was carried out using IHOP; genes were classified according to the conceptual diagram «Gene Ontology» (GO) and were ranked according to  their first priority for the experimental validation.
On the basis of conducted analysis 162 genes of potential primary response to ІFN? were subdivided into two groups – 61 genes, for which the experimental data of their responsibility to IFN? were found and 101 genes for which such data are absent. We have performed the functional analysis of the «unknown» genes according to GO. The enriched category «Synapse part» encountering  three genes of Rattus norvegicus: PRKCA-binding­ of protein, NMDAR-L and GABAA – receptor subunit alpha-2 were revealed. Among 162 genes the enriched category «Immune response» was detected with Mbl1 gene, which is reckoned among «unknown» genes. The four designated genes are chosen as the candidates for the prior validation.

Key words: interferon alpha, genes of potential response, classification of genes of response, «Gene Ontology», FatiGO, GOTM.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal