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Journal archive > 2009 > N 6 November-December

INFLUENCE OF GOLD AND SILVER NANOPARTICLES ON АТРase ACTIVITY
OF NATIVE AND REHYDRATED CELLS OF Escherichia coli

 

М. E. Roman’ko1, L. S. Rieznichenko2,3, T.G.Gruzina2, S. M. Dybkova2,
Z.R.Ulberg2, V. O. Ushkalov3, A.M.Golovko3

 

1National Scientific Centre «Institute of Experimental and Clinical Veterinary Medicine», Ukrainian Academy of Agrarian Sciences, Kharkiv;
2Ovcharenko Institute of Biocolloidal Chemistry, National Academy of Sciences of Ukraine, Kyiv;
3The State Science-Control Institute of Biotechnology and Strains of Microorganisms, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The expressed changes in the bacteria cells’ plasma membrane ATPase activity values cells of Escherichia coli, which are used in the manufacturing of vaccines against cattle colientero­toxemia, have been revealed under the influence of lyophilization/rehydration process.
Influence of gold (average size 20, 30, 45?nm) and silver (30 nm) nanoparticles in different concentrations on ATPase activity of native and rehydrated (delyophilized) bacteria cells has been studied.
Gold and silver nanoparticles stimulated ATPase­ activity of native and rehydrated (delyophilizated) cells of the investigated strains.
Obtained data show, that application of exa­mined metal nanoparticles of certain sizes and concentrations as protectors and stimulators of main physiological-biochemical properties of production strains’ cells under the conditions of lyophilization and long storage stress in technologies of different immunobiological preparations manufacturing is very promising.

Key words: production bacteria strains, lyophilization, rehydration, nanoparticles, gold, silver, ATPase activity.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal