V. S. Asmolkova, G. I. Lavrenchuk, Т. О. Khmel, N. М. Gula
Palladin Institute of Biochemistry, National Academy of Scienсes, Ukraine, Kyiv;
The effects of the N-stearoylethanolamine (NSE), N-oleoylethanolamine (OEA) and N-acylethanolamine (NAE) mixture on the cell survival, apoptosis and activity of mitochondrial succinate dehydrogenase (SDG) and glycerophosphate dehydrogenase (GFDG) in embryonic cell culture under normal conditions and irradiation were compared in the work.
It was shown, that all investigated NAE were able to modulate the proliferative activity of intact cells as well as irradiation-exposed cells in concentration-depended manner. The most pronounced effect was observed under the NAE mixture action. It was established, that NAE prevented the damage effects of the irradiation and diminished the activation of apoptotic cell death. It was foud that NSE and OEA decreased the activity of the SDG (42.3 and 44.14%, accordingly) and the GFDG activity (14.67 and 17.33%, accordingly) in embryonic cell culture, while addition of the NAE mixture decreased SDG activity by 20%, at the same time GFDG activity increased by 20%.
Our findings suggested that antiproliferative effects of NAE depended on their influence on mitochondrial functioning.
Key words: N-acylethanolamines, proliferative activity, apoptosis, mitochondrial enzymes, ionizing irradiation.
The original article in Ukrainian is available for download in PDF format.
© The Ukrainian Biochemical Journal