L. P. Kuznetsova, E. R. Nikitina, E. E. Sochilina, K. A. Vasiljeva
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of?Sciences, Sankt-Petersburg, Russia;
The influence of some cationic detergents on the catalytic activity of the horse blood plasma cholinesterase in reaction of hydrolysis of ?-naphthylacetate at different рН were investigated. It was shown, that in the absence of detergents in acid рН of the reaction medium the Кm value increases, but V remain constant. In the range of рН from 8.5 to 5.0 in the presence of detergents the Кm and V values are not practically changed. That is why the activation of cholinesterase hydrolysis of ?-naphthylacetate in the presence of detergents is considerably higher than that of the neutral pH.
Key words: BuCHE of horse blood plasma, enzymatic hydrolysis, ?-naphthylacetate, activation, cetylpyridinium, cetyltrimethylammonium.
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