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Journal archive > 2009 > N 4 July-August

THE INFLUENCE OF рН ON CHOLINESTERASE HYDROLYSIS OF ?-NAPHTHYLACETATE
IN THE PRESENCE OF SOME CATIONIC DETERGENTS

L. P. Kuznetsova, E. R. Nikitina, E. E. Sochilina, K. A. Vasiljeva

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of?Sciences, Sankt-Petersburg, Russia;
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The influence of some cationic detergents on the catalytic activity of the horse blood plasma cholinesterase in reaction of hydrolysis of ?-naphthylacetate at different рН were investigated. It was shown, that in the absence of detergents in acid рН of the reaction medium the Кm value increases, but V remain constant. In the range of рН from 8.5 to 5.0 in the presence of detergents the Кm and V values are not practically changed. That is why the activation of cholinesterase hydrolysis of ?-naphthylacetate in the presence of detergents is conside­rably higher than that of the neutral pH.

Key words: BuCHE of horse blood plasma, enzymatic hydrolysis, ?-naphthylacetate, activation, cetylpyridinium, cetyltrimethylammonium.

The original article in Russian is available for download in PDF format.

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