N. V. Naumova, L. G. Babich, S. G. Shlykov
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
Using the method of flow cytometry and potential-sensitive fluorescent dye TMRM the effect of divalent cations on the membrane potential of isolated myometrium mitochondria was studied. It was shown, that Mg2+ (7 mM) addition to the incubation medium induced mitochondrial membrane hyperpolarization. In the case of protonophore CCCP (1 µM) preliminary presence in the incubation medium, Mg2+ addition did not lead to membrane potential hyperpolarization. Addition of Ca2+ leads to membrane potential dissipation. Ca2+-induced mitochondrial depolarization is the same as NaN3 (10 mM) - induced depolarization. It also was shown that Mg2+ (7 mM) preliminary presence in the incubation medium did not protect mitochondria from Ca2+-induced depolarization.
Key words: flow cytometry, isolated mitochondria, mitochondrial membrane potential.
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