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Journal archive > 2009 > N 4 July-August

HYDROGEN PEROXIDE INHIBITS ACETYLCHOLINESTERASE OF MYOMETRIUM SARCOLEMMA

Iu. V. Danylovych

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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The action of hydrogen peroxide on acetylcholinesterase enzymatic activity in myometrium sarcolemma fraction is investigated. Hydrogen peroxide (0.1–26 µM), depending on the concentration, suppressed the activity. Acetylcholinesterase proved to be highly sensitive to the action of H2O2, making Ki = 2.4 ± 0.4 µM, nH = 0.65 ± 0.08 (n?=?4–5). It is established, that hydrogen peroxide in the range of 1.6–6.4 µM essentially reduce V0,max and KM. In the presence of dithiothreitole (a reducer of SH-groups of the membrane surface) the investigated substance effect considerably decreased.

Key words: myometrium, hydrogen peroxi­de, acetylcholinesterase.

The original article in Ukrainian is available for download in PDF format.

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