DETECTION OF ANTIADENOVIRAL ANTIBODIES BY SURFACE PLAZMON REZONANCE
L. M. Nosach1, P. M. Boltovets1, S. D. Zagorodnya1, O. Y. Povnitsa1, A. V.?Golovan1, N. I. Netreba2, L.?I.?Dobrochinska2
1Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Pediatrics, Obstetrics and Gynecology, AMS of Ukraine Kyiv;
A possibility to detect antiadenoviral antibodies by surface plasmon resonance (SPR) was demonstrated. Immobilization on the surface of a sensor of the hexone or degraded purified adenovirus of one of the types (Аd 2) allows finding antibodies to the hexone antigenic determinants of wide specificity common for human adenoviruses of different types. Optimum conditions for immobilization of the antigen and formation of the complex antigen-antibody are determined. The comparative assays of the levels of antibodies in rabbit antisera obtained to the hexone and adenoviruses of different types (1, 2 and 6) by SPR and ELISA was analyzed. The biosensor was used to detect antiadenoviral antibodies in the blood sera of children with aggravation of chronic nonspecific broncho-pulmonary diseases. The sensitivity of SPR in comparison with ELISA was 86.9%, in comparison with the method of fluorescing antibodies (MFA)?– 89.5%.
Key words: adenovirus, hexone, SPR, ELISA, MFA, antihexone and antiadenoviral serums, the blood serums of patients.
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© The Ukrainian Biochemical Journal