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Journal archive > 2009 > N 4 July-August

EXPRESSION OF 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE-3
AND VEGF mRNA IN RAT LIVER, LUNG AND HEART: EFFECT OF METHYL TERTBUTYL ETHER

D. O. Minchenko1, A. V. Kundieva1, K. Tsuchihara2, O. P. Yavorovsky3, Y. O. Paustovsky3, H. Esumi2, O. H. Minchenko1,2*

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine;
2Research Center for Innovative Oncology, National Cancer Center Hospital, East Kashiwa, Japan;
3Bohomolets National Medical University, Kyiv, Ukraine;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The main goal of this work was investigation of the effect of methyl tertbutyl ether, ecologically dangerous chemical compound, on the expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB-3) and vascular endothelial growth factor (VEGF) mRNA in different rat organs. Expression of PFKFB-3 and VEGF is a hypoxia inducible factor (HIF)-dependent process which significantly increases under hypoxia, in malignant tumors and other pathology. In this study we have shown that PFKFB-3 and VEGF mRNA expression in the liver, lung, and heart changes in rats, treated with methyl tertbutyl ether for two months, in organ-specific manner. Expression of alternative splice variants of PFKFB-3 mRNA as well as VEGF mRNA also changes in organ-specific manner in rats, treated with methyl tertbutyl ether. The effect of methyl tertbutyl ether on the expression of PFKFB-3 and VEGF mRNA and its alternative splice variants is dose-dependent. Results of this investigation clearly demonstrated that methyl tertbutyl ether affects the expression of PFKFB-3, a key regulatory enzyme of glycolysis, as well as VEGF, very important factor of angiogenesis, in an organ-specific and dose-dependent manner.

Key words: PFKFB-4 mRNA, alternative splicing, lung, liver, heart, methyl tertbutyl ether, rats.

The original article in English is available for download in PDF format.

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