1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2National Institute of Cancer of Ministry of Public Health, Kyiv, Ukraine;
3Tumor and Cell Biology Center, Karolinska Institute, Stockholm, Sweden;
DNA microarray technology comprising NotI-linking clones was used in a large-scale study of genetic and epigenetic changes in colorectal cancer. Analysis of samples from 24 patients revealed methylation, deletions, and amplifications in 137 of 181 NotI clones. For 27 genes/loci, these changes occurred in more than 30% of the tumor samples, suggesting that these genes are involved in the development of colorectal cancer. An analysis of the methylation status of CpG island of the ITGA9 gene/loci by bisulfite sequencing confirmed the NotI microarray data on the gene/loci methylation in colorectal cancer. Aberrations in 19 genes/loci were unknown previously. Their characterization may help ascertain the mechanisms responsible for colorectal cancer development and identify novel diagnostic and prognostic markers.
Key words: NotI microarrays, human chromosome 3, DNA methylation, colorectal cancer.
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