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Journal archive > 2009 > N 4 July-August

SCREENING OF EPIGENETIC AND GENETIC DISTURBENCES OF HUMAN
CHROMOSOME 3 GENES IN COLORECTAL CANCER
 
 
 
G. V. Gerashchenko1, V. V. Gordiyuk1, I. Ya. Skrypkina1, S. M. Kvasha1, O. O. Kolesnik2,
D. D. Ugryn1, T. V. Pavlova3, E. R. Zabarovsky3, A. V. Rynditch1, V. I. Kashuba1,3

1Institute of Molecular Biology and Genetics, National Academy of Sciences  of Ukraine, Kyiv;
2National Institute of Cancer of Ministry of Public Health, Kyiv, Ukraine;
3Tumor and Cell Biology Center, Karolinska Institute, Stockholm, Sweden;
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DNA microarray technology comprising NotI-linking clones was used in a large-scale study of genetic and epigenetic changes in colorectal cancer. Analysis of samples from 24 patients revealed methylation, deletions, and amplifications in 137 of 181 NotI clones. For 27 genes/loci, these changes occurred in more than 30% of the tumor samples, suggesting that these genes are involved in the development of colorectal cancer. An analysis of the methylation status of CpG island of the ITGA9 gene/loci by bisulfite sequencing confirmed the NotI microarray data on the gene/loci methylation in colorectal cancer. Aberrations in 19 genes/loci were unknown previously. Their characterization may help ascertain the mechanisms responsible for colorectal cancer development and identify novel diagnostic and prognostic markers.

Key words: NotI microarrays, human chromosome 3, DNA methylation, colorectal cancer.

The original article in English is available for download in PDF format.

© The Ukrainian Biochemical Journal