ANTISENSE OLIGONUCLEOTIDES AS POTENTIAL DRUGS FOR?PROPHYLAXIS OF PRION INFECTIONS
V. V. Stadnyk1, L. A. Izyumova1, Yu.?A.?Rzhepeckyy2, Ch. Ya. Mayor1, P.?I.?Verbitskyy1, V. V. Vlizlo1
1Institute of Animal Biology Ukrainian Academy of Agrarian Sciences, Lviv, Ukraine;
2Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
Prion mRNA translation inhibition by antisense oligodeoxynucleotides (asODN) incorporated into immunoliposomes was investigated. It was shown that asODN complementary to cap region, start-codon region and a part of open reading frame can decrease the prion expression by 80% in L1210 cell line and by 60% in prion-replicating organs of laboratory rats. These results give grounds for further research asODN to be used as a means of prevention and treatment of prion infections.
Key words: cellular prion, antisense oligodeoxynucleotides, immunoliposomes, prion infections prevention.
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