L. V. Porubleva1, А. V. Rebriiev2, T. Yu. Gromovoy3, I. J. Minia1, M. Yu. Obolenskaya1
1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3Chuyko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kyiv;
MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometry has become, in the recent years, a tool of choice for analyses of biological polymers. The wide mass range, high accuracy, informativity and sensitivity make it a superior method for analysis of all kinds of high-molecular biological compounds including proteins, nucleic acids and lipids. MALDI-TOF MS is particularly suitable for the identification of proteins by mass fingerprint or microsequencing. Therefore it has become an important technique of proteomics. Furthermore, the method allows making a detailed analysis of post-translational protein modifications, protein-protein and protein-nucleic acid interactions. Recently, the method was also successfully applied to nucleic acid sequencing as well as screening for mutations.
Key words: MALDI-TOF mass spectrometry, protein identification, peptide mass fingerprints.
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