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Journal archive > 2009 > N 3 May-June

MALDI-TOF MASS SPECTROMETRY IN THE INVESTIGATION OF LARGE
HIGH-MOLECULAR BIOLOGICAL COMPOUNDS

L. V. Porubleva1, А. V. Rebriiev2, T. Yu. Gromovoy3, I. J. Minia1, M. Yu. Obolenskaya1

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3Chuyko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kyiv;
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MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometry has become, in the recent years, a tool of choice for analyses of biological polymers. The wide mass range, high accuracy, informativity and sensitivity make it a superior method for analysis of all kinds of high-molecular biological compounds including proteins, nucleic acids and lipids­. MALDI-TOF MS is particularly suitable for the identification of proteins by mass fingerprint or microsequencing. Therefore it has become an important technique of proteomics. Furthermore, the method allows making­ a detailed analysis of post-translational protein modifications, protein-protein and protein-nucleic acid interactions. Recently, the method was also successfully applied to nucleic acid sequencing as well as screening for mutations.

Key words: MALDI-TOF mass spectro­met­ry, protein identification, peptide mass fingerprints.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal