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Journal archive > 2009 > N 3 May-June

SHORTCOMINGS OF TRADITIONAL METHOD FOR DETERMINING AFFINITY AND THEIR REMOVAL

S. A. Bobrovnik, M. A. Demchenko, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev;
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It was shown that application of the traditional­ method for antibody affinity determination may give not exact and non complete information about the characteristics of studied antibodies especially if studying specimens contain two or more species of antibodies with different affinity.
In order to avoid this problem it is recommended to use another approach, suggested by us earlier.
Our approach allows elucidating that studying­ specimens contain more than one kind of antibodies­ and this allows applying a proper method for antibody affinity evaluation. Such method, which allows determining the affinity of two antibodies in a mixture that have different affinity, is also described.

Key words: antigen-antibody reaction, affinity, mathematical modeling.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal